569 papers
This study reveals that chromosome condensation mechanically primes the nucleus for mitosis by increasing nuclear envelope tension, a process dependent on SUN proteins that facilitates cyclin B1 translocation and dynein loading to ensure timely mitotic entry and prevent segregation errors.
This study combines GlycoSHIELD computational modeling and DNA-PAINT super-resolution microscopy to demonstrate that N-glycosylation of the Na,K-ATPase β1 subunit promotes stable nanoscale clustering via a galectin-lattice mechanism rather than steric repulsion.
This study utilizes longitudinal single-nucleus profiling and lineage tracing to identify a transient PAX8+ intermediate mesoderm niche that supports early human myogenesis through BMP7-laminin signaling and SIX1-EYA3 cofactor switching, thereby defining the intrinsic and extrinsic mechanisms governing the PAX3-to-PAX7 progenitor transition.
This study validates SERPINB2 as a robust, cross-platform chondrogenic biomarker and reveals a coordinated activation of the SERPIN network during cartilage lineage commitment, highlighting its potential role in regulating the chondro-osteogenic decision and its utility for osteoarthritis monitoring.
This study identifies Rab12 as a novel negative regulator of mitophagy and mitochondrial homeostasis, demonstrating that its depletion enhances the clearance of damaged mitochondria and links vesicular trafficking machinery to mitochondrial quality control with potential implications for neurodegenerative diseases.
This study demonstrates that extracellular vesicles derived from small airway epithelial cells and fibroblasts in COPD patients carry distinct microRNA signatures enriched in pathways driving cellular senescence and fibrosis, suggesting their critical role in disease progression.
This study identifies 17 proteins localizing to the complex feeding and flagellar apparatus of the marine heterotrophic flagellate *Diplonema papillatum*, including novel components like Mad2 and MBP65, thereby establishing a molecular foundation for understanding nutrient acquisition mechanisms in this abundant group of plankton.
This study identifies the ER-resident protein MOSPD2 as a selective recruiter of the cholesterol transporter STARD3 at ER-late endosome/lysosome contact sites, establishing their specific interaction as a unique functional unit essential for maintaining lysosomal dynamics and cholesterol homeostasis.
This study reveals that denatured serum albumin, induced by physiological conditions like alcohol intake or oxidative stress, binds to platelet integrin IIb3 to regulate activation, adhesion, and aggregation as effectively as fibrinogen, thereby identifying a previously unrecognized mechanism for thrombotic risk.
This study demonstrates that VeritaCell-derived autologous skin cell suspensions significantly accelerate wound healing and reduce scarring-associated thickening in a rat model, validating their efficacy even at low donor-to-wound ratios and suggesting a promising strategy for treating extensive injuries like burns while minimizing donor skin requirements.
This study reveals that importin-beta (Impβ), traditionally known for mediating nucleocytoplasmic transport, directly regulates actin polymerization and stress fiber formation to control cell migration and tissue integrity, a function distinct from its canonical transport role.
This study reveals that SMN deficiency in Spinal Muscular Atrophy drives extensive, tissue-specific proteomic remodeling across multiple organs, with partial SMN restoration via antisense oligonucleotides only incompletely reversing these molecular alterations, particularly in metabolic and mitochondrial pathways.
This study demonstrates that PARP16 protects against cardiac hypertrophy and heart failure by ADP-ribosylating and inhibiting the transcriptional activity of NFAT1, a mechanism whose loss leads to pathological cardiac remodeling.
This study demonstrates that inducible deletion of endothelial KLF4 in mice recapitulates the key clinical features of preeclampsia, including gestational hypertension and fetal growth restriction, thereby providing a novel model that links upstream cardiovascular risk factors to the disease's underlying mechanisms.
This study reveals that *Tetrahymena thermophila* responds to calcium-induced stress by internalizing motile cilia into ring-like structures that undergo regulated bulk degradation via autophagy, a process termed "motile ciliophagy," to recycle axonemal components for efficient ciliary regeneration and cellular homeostasis.
This study identifies the ubiquitin ligase CHFR as a critical regulator of endothelial barrier dysfunction during inflammation by mediating the K48-linked ubiquitylation and proteasomal degradation of Akt1, thereby disrupting the Ang-1/Tie2 signaling axis and promoting VE-cadherin loss.
This study employs an image-based granularity screening approach to identify specific vaccinia virus membrane proteins that induce LC3 lipidation, revealing a more directed interplay between the virus and the host autophagy pathway than previously understood.
This study demonstrates that the transcription factor ATF4 coordinates transcriptomic and structural adaptations in aging muscle by regulating the integrated stress response, which drives mitochondrial dysfunction and sarcopenia, thereby identifying the ISR pathway as a potential therapeutic target for preserving muscle function in older adults.
This study introduces fishROI, a specialized FIJI2 plugin that combines cytoplasmic staining with deep learning to provide a robust, semi-automated workflow for analyzing and visualizing the unique muscle morphometry and hyperplastic growth dynamics of teleosts.
This paper presents a streamlined protocol for rapid CRISPR-Cas9 genome editing in *Saccharomyces cerevisiae* that utilizes PCR-based protospacer installation and seamless plasmid assembly to bypass traditional restriction/ligation cloning, followed by LiAc/PEG co-transformation and PCR-based verification of edits.